KMID : 0894520170210020205
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Development & Reproduction 2017 Volume.21 No. 2 p.205 ~ p.213
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Effect of Alpha-Linolenic Acid on Oocyte Maturation and Embryo Development in Pigs
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Lee Ji-Eun
Hwangbo Yong Kim Hwa-Young Lee Won-Hee Cheong Hee-Tae Yang Boo-Keun Park Choon-Keun
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Abstract
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The aim of this study was to determine the effect of additional alpha-linolenic acid (ALA) supplementation during in vitro maturation (IVM) and culture (IVC) on nucleic maturation and embryo development of pigs. Cumulus-oocyte complexes (COCs) were incubated in IVM medium containing different concentration of ALA (25, 50 and 100 ¥ìM) for 44 h. After in vitro maturation, nuclear maturation of oocytes were evaluated by aceto-orcein stain. Mature oocytes with 50 ¥ìM ALA were fertilized and cultured in IVC medium with ALA (25, 50 and 100 ¥ìM) during early-embryogenesis (48 hours after fertilization). Then, embryos were cultured with 25 ¥ìM ALA during early embryogenesis and/or late embryogenesis (120 hours after early-embryogenesis). In results, oocyte maturation were significantly increased by 50 ¥ìM ALA treatment groups compared with control groups (p<0.05). Treatment of 25 ¥ìM ALA during early-embryogenesis enhanced cleavage rate of em-bryo compared with other groups (p<0.05), whereas formation and total cell number of blastocyst had no significant differ-ence. Similarly, cleavage rate of embryos were increased by 25 ¥ìM ALA supplement during early- or late-embryogenesis than ALA treatment both stage of embryogenesis (p<0.05), but did not influence to blastocyst formation. Interestingly, total cell number of blastocyst were enhanced in ALA treatment group during early-embryogenesis. These findings indicated that ALA supplement enhance the nuclear maturation of oocyte and embryo development, however, excessive ALA could negatively in-fluence. Therefore, we suggest that ALA is used for improvement of in vitro production of mammalian embryo and further study regarding with functional mechanism of ALA is needed.
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KEYWORD
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Alpha-linolenic acid , In vitro maturation , In vitro culture , Oocyte , Embryo , Pig
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